Semantic resources project/Antibodies/Revised Methods/Immunodiffusion

Description
ID: "Immunodiffusion"
 * aka. Agar Gel Immunodiffusion (AGID)

Outline
A qualitative assay used to determine the presence of an antigen by allowing both antigen and antibody to diffuse through a gel.

The presence, thickness, and location of the precipitin line through the gel are the assay's report.

Ontology Terms
MeSH: "Immunodiffusion"
 * D005779
 * http://bioportal.bioontology.org/visualize/40401/D005779
 * Technique involving the diffusion of antigen or antibody through a semisolid medium, usually agar or agarose gel, with the result being a precipitin reaction.

HL7v3.0: "Immunodiffusion"
 * 0063
 * http://bioportal.bioontology.org/visualize/40392/0063

SNOMED: "Immunodiffusion measurement"
 * 32623009
 * http://bioportal.bioontology.org/visualize/40403/32623009

Usage
Maheswari et al. "Isolation and characterization of an acetyl group-recognizing agglutinin from the serum of the Indian white shrimp Fenneropenaeus indicus". Archives of Biochemistry and Biophysics 402:1,65-76. (June 2002) "'To examine the reactivity of the antiserum against FIA and whole serum of F.indicus by double immunodiffusion (ID) and immunoelectrophoresis (IEP), anti-FIA serum as well as preimmune rabbit serum were fivefold diluted with TBS-II. In ID analyses, 15ll of the sample to be tested was applied to 1% agarose gel prepared in 25 mM borate-buffered saline (pH 8.5) following Ouchterlony. The samples were then allowed to diffuse for 72 h at 24 °C to develop precipitation lines.'"

Definition
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