Semantic resources project/Antibodies/Revised Methods/EMSA

Description
EMSA: Electrophoretic Mobility Shift Assay

Ontology Terms
MeSH: "Electrophoretic Mobility Shift Assay"
 * D024202
 * http://bioportal.bioontology.org/visualize/40401/D024202
 * "An electrophoretic technique for assaying the binding of one compound to another. Typically one compound is labeled to follow its mobility during electrophoresis. If the labeled compound is bound by the other compound, then the mobility of the labeled compound through the electrophoretic medium will be retarded."

Protein-protein interaction: "electrophoretic mobility shift assay"
 * MI:0413
 * http://bioportal.bioontology.org/visualize/39508/MI%253A0413
 * "This method proves the interaction between a nucleic acid and a protein partner. On the same electrophoresis gel 1 lane is loaded with a radiolabeled nucleic acid of known sequence, a second lane is loaded with the same nucleic acid together with a purified protein (or a protein mixture). After the electrophoresis run and autoradiography by comparing the nucleic acid migration in the two lanes the retardation of the nucleic acid due to its interaction with a protein can be easily observed."

NCI Thesaurus: "Electrophoretic Mobility Shift Assay"
 * http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#Electrophoretic_Mobility_Shift_Assay
 * http://bioportal.bioontology.org/visualize/40644/Electrophoretic_Mobility_Shift_Assay
 * "A molecular biology technique used to detect the interaction of a DNA binding protein with its cognate binding sequence. Labeled DNAs were reacted with crude cell extracts and the complexes are run through a non-denaturing polyacrylamide gel. The migration of the labeled DNA through the gel will be slower by being bound. This shift in electrophoretic mobility indicates functional binding between the protein and the DNA."

Usage
Chernov et al. Identification of tissue-specific DNA–protein binding sites by means of two-dimensional electrophoretic mobility shift assay display. Analytical Biochemistry 364:1,60-66 (May 2007) "'We developed a technique of differential electrophoretic mobility shift assay (EMSA) display allowing identification of tissue-specific protein-binding sites within long genomic sequences. Using this approach, we identified 10 cell type-specific protein-binding sites (protein target sites [PTSs]) within a 137-kb human chromosome 19 region.'"

Definition
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