Semantic resources project/Antibodies/Revised Methods

= Representing antibody creation and use =

Assay types and antibody manufacturing are in the domain of Ontology for Biomedical Investigations (OBI) The work below is preparatory to a submission request for any assays that use Antibodies that are not already in OBI. For new submissions our job is to prepare the minimal metadata about the assay, to make it easy for OBI to satisfy the term request.

Assays and Experiment descriptions

 * Immunochemistry
 * Immunohistochemistry
 * In-situ Hybridization (ISH)
 * Fluorescent In-situ Hybridization (FISH)
 * Immunocytochemistry
 * Immunoassay
 * Nephelometric Immunoassay (NIA)
 * Turbidimetric Immunoassay (TIA)
 * Competitive Immunoassay
 * Noncompetitive Immunoassay
 * Immunofluorescence (IF)
 * Fluorescent In-situ Hybridization (FISH)
 * Enzyme-linked Immunofluorescent Assay (ELIFA)
 * Radio Immunoassay (RIA)
 * Magnetic Immunoassay
 * Enzyme-linked Immunosorbant Assay (ELISA)
 * ELISPOT
 * Indirect ELISA
 * Sandwich ELISA
 * Competitive ELISA
 * Reverse ELISA
 * Immunodiffusion (ID)
 * Double Immunodiffusion (DID)
 * Immunostaining (IS)
 * Blotting
 * Western Blot/Immunoblotting
 * Far-Western Blot
 * Northern Blot
 * Southern Blot
 * Southwestern Blot
 * Eastern Blot
 * Far-Eastern Blot
 * Immunoprecipitation (IP)
 * Complex Immunoprecipitation (Co-IP)
 * Cross-linking and Immunoprecipitation (CLIP)
 * Chromatin Immunoprecipitation (ChIP)
 * Radio Immunoprecipitation (RIP)
 * Chromatography
 * Affinity Chromatography (AC)
 * Electrophoresis
 * Capillary Electrophoresis (CE)
 * Gel electrophoresis (GE)
 * Electrophoretic Mobility Shift Assay (EMSA)
 * 2D Gel Electrophoresis (2DE)
 * Immunoelectrophoresis
 * Immunofixation Electrophoresis (IFE)
 * Countercurrent Immunoelectrophoresis (CIE)
 * Crossed Immunoelectrophoresis (Crossed IEP)
 * Rocket Immunoelectrophoresis (RIEP)
 * Polyacrylamide Gel Electrophoresis (PAGE)
 * SDS-PAGE
 * Native PAGE
 * Blue Native PAGE
 * Clear Native PAGE
 * Quantitative Preparative Native Continuous PAGE
 * Antigen Retrieval
 * Antigen Assay
 * B-Cell Assay
 * T-Cell Assay
 * Microarrays
 * Tissue Microarray (TMA)
 * Affinity Microarrays (AM)
 * Microscopy
 * Confocal Microscopy (CM)
 * Flow Cytometry (FC or FACS)

Materials and Reagents

 * Magnetic Beads (MB)
 * Multiplex Arrays (MxA)
 * Membrane
 * PVDF Membrane
 * Nitrocellulose Membrane
 * Protein
 * Immunoglobulin-binding Protein
 * Protein A
 * Protein G
 * Stain
 * Silver staining
 * Coomassie
 * Activity Stains? (natural vs. denatured proteins)

Outstanding Issues
How do I characterize something like "antigen retrieval?"

Should I be thinking about reagents (as listed below) in addition to experimental processes?

How do I resolve situations like PAGE, which is sometimes used for proteins and sometimes for nucleic acids? (Clearly, it's not a subset of "Immunoelectrophoresis.")

When should I try to adapt MeSH or SNOMED definitions, where available?

Antibody Creation
Cell fusion

Mouse myeloma cells

blood lymphocytes

monoclonal,polyclonal

immunization

titer

adjuvant

Links
OpenWetware's "Generating Antibodies" page