Semantic resources project/Antibodies/Revised Methods/Immunohistochemistry

Names
Immunohistochemistry

Outline
Immunochemistry on tissue samples (as opposed to cell samples).

Ontology Terms
MeSH: "Immunohistochemistry"
 * D007150
 * http://bioportal.bioontology.org/visualize/40401/D007150
 * "Histochemical localization of immunoreactive substances using labeled antibodies as reagents."

NCI Thesaurus: "Immunohistochemistry"
 * http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#Immunohistochemistry
 * http://bioportal.bioontology.org/visualize/40377/Immunohistochemistry
 * Retired Concept
 * "An assay that shows specific antigens by the use of markers that are either fluorescent dyes or enzymes. In these techniques an antibody is used to link a cellular antigen specifically to a stain that can be more readily seen with a microscope. Detection of antigens in tissues is known as immunohistochemistry, while detection in cultured cells is generally termed immunocytochemistry."

NIFSTD: "Immunohistochemistry"
 * http://ontology.neuinfo.org/NIF/DigitalEntities/NIF-Investigation.owl#nlx_inv_20090609
 * http://bioportal.bioontology.org/visualize/40510/p17%253Anlx_inv_20090609
 * "Immunolabeling protocol application that involves localizing proteins in a tissue section through exploiting the principle of antibodies binding specifically to antigens in biological tissues. It takes its name from the roots "immuno," in reference to antibodies used in the procedure, and "histo," meaning tissue (compare to immunocytochemistry). (from wikipedia)"

Usage
"'From the beginning immunohistochemistry has been adapted to provide a range of markers of cell lineage and tissue type, with particular application to the diagnosis and classification of tumours. ... This review explores the limitations of immunohistochemistry when employed in a semiquantitative mode, and explores the possibility of fulfilling the full potential of immunohistochemistry, as a true quantitative immunoassay applied in a tissue section environment.'"

From Taylor and Levenson, "Quantification of immunohistochemistry—issues concerning methods, utility and semiquantitative assessment II". Histopathology 49:4,411-424. (September 2006)

Definition
First attempt, adapted from the NCI Thesaurus Definition

An immunoassay in which the bound antigen is present as part of a tissue slice or section, rather than located on individual cells or within a processed sample.