Semantic resources project/Antibodies/Revised Methods/Native PAGE

Description
Native PAGE

Outline
Native Polyacrylamide Gel Electrophoresis -- that is, gel electrophoresis of proteins without an initial "denaturing" step. This affects the ability of the antibody to recognize the antigen, which will be present (in general) in either a "linear" form (in a denatured protein) or in its native conformation (natural proteins).

SDS, used in SDS-PAGE, is a detergent which denatures the proteins.

Ontology Terms
Protein-protein Interaction: "blue-native page"
 * MI:0276
 * http://bioportal.bioontology.org/visualize/39508/MI%253A0276
 * "Blue native PAGE (BN-PAGE) permits a high-resolution separation of multi-protein complexes under native conditions. Blue native (BN)-PAGE is a charge shift method, in which the electrophoretic mobility of a complex is determined by the negative charge of the bound Coomassie dye and the size and shape of the complex. Coomassie does not act as a detergent and preserves the structure of complexes. Importantly, the resolution of BN-PAGE is much higher than that of other methods such as gel filtration or sucrose-gradient ultracentrifugation. Combined with other pre-purifications or dialysis steps this method permits the analysis of multi-protein complexes of whole cellular lysates by BN-PAGE."
 * Obviously, this is a type of Native PAGE -- some of the superclasses of this term may be useful here too

Definition
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