Semantic resources project/Antibodies/Revised Methods/Antigen Retrieval

Description
Antigen Retrieval

Outline
Some staining procedures (immunohistochemistry) require fixing the target using parafin or formalin or other crosslinking agents. However, a subsequent step is then required to "reveal" the antigens in order to get the antibody-antigen reaction -- this is the antigen retrieval step.

Ontology Terms
None that I can find

Usage
Tang et al. Antigen-Retrieval Procedure for Bromodeoxyuridine Immunolabeling with Concurrent Labeling of Nuclear DNA and Antigens Damaged by HCl Pretreatment. The Journal of Neuroscience 27:22,5837-5844 (May 2007) "'Before adopting the steamer, we had performed heat-induced epitope retrieval using a microwave oven to reveal p27Kip1 immunoreactivity. Although the microwave pretreatment protocol used for p27Kip1 (referred to below as “low energy”) was ineffective in revealing BrdU, we serendipitously discovered that more intense microwave pretreatment (referred to below as “high energy”) did reveal BrdU.'"

Definition
 None yet