Semantic resources project/Antibodies/Revised Methods/Affinity Chromatography

Description
AC: Affinity Chromatography

Ontology Terms
MeSH: "Chromatography, Affinity"
 * D002846
 * http://bioportal.bioontology.org/visualize/40401/D002846
 * "A chromatographic technique that utilizes the ability of biological molecules to bind to certain ligands specifically and reversibly. It is used in protein biochemistry. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)"

NCI Thesaurus: "Affinity Chromatography"
 * Affinity_Chromatography
 * http://bioportal.bioontology.org/visualize/40644/Affinity_Chromatography
 * "A chromatographic separation technique based on the ability of proteins to bind specific ligands reversibly."

Usage
Yatohgo et al. "Novel Purification of Vitronectin from Human Plasma by Heparin Affinity Chromatography". Cell Structure and Function 13:4,281-292 (1988) "We developed a strikingly simple method to purify vitronectin from human plasma by heparin affinity chromatography. Serum was obtained from plasma by adding calcium and then centrifugating. The heparin-binding activity of vitronectin in human serum was activated with 8 M urea.  The activated vitronectin specifically bound to heparin-Sepharose in 8 M urea and was eluted with 0.5 M NaCl containing 8 M urea.  This procedure resulted in an approximately 250-fold purification of vitronectin with a 15-30% recovery."

Definition
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