Semantic resources project/Antibodies/Revised Methods/Cross-linking and Immunoprecipitation

Description
CLIP: Cross-linking and Immunoprecipitation

Ontology Terms
SNOMED: "Cross-linking"
 * 85952003
 * http://bioportal.bioontology.org/visualize/40403/85952003

ChEBI: "Cross-linking reagent"
 * CHEBI:50684
 * http://bioportal.bioontology.org/visualize/40664/CHEBI%253A50684
 * A reagent with two reactive groups, usually at opposite ends of the molecule, that are capable of reacting with and thereby forming bridges between macromolecules, principally side chains of amino acids in proteins, allowing the locations of naturally reactive areas within the proteins to be identified.

Usage
Licatalosi et al. "HITS-CLIP yields genome-wide insights into brain alternative RNA processing". Nature 456,464-469 (November 2008) "'We studied Nova–RNA interactions in the mouse neocortex, which expresses the Nova2 protein. We identified 2,481 Nova-bound RNAs (CLIP tags) from five experiments using traditional CLIP strategies and 412,686 CLIP tags from three experiments using high-throughput pyrosequencing.'" This paper also contains a reasonable high-level definition of CLIP. (HITS-CLIP is a variant of CLIP that we might want to possibly model.)