Semantic resources project/Antibodies/Revised Methods/2D-Gel Electrophoresis

Description
2DE: 2-D Gel Electrophoresis

Outline
A sequential technique, not restricted to either proteins or nucleic acids.

Ontology Terms
MeSH: "Electrophoresis, Gel, Two-Dimensional"
 * D015180
 * http://bioportal.bioontology.org/visualize/40401/D015180
 * "Electrophoresis in which a second perpendicular electrophoretic transport is performed on the separate components resulting from the first electrophoresis. This technique is usually performed on polyacrylamide gels."

Usage
Haqquani et al. Protein Markers of Ischemic Insult in Brain Endothelial Cells Identified Using 2D Gel Electrophoresis and ICAT-Based Quantitative Proteomics. Journal of Proteome Research 6:1,226–239 (2007) "'The only published application of proteomics to analyze BEC responses to hypoxia and posthypoxic reoxygenation identified changes in abundant cellular proteins including glycolytic enzymes, endoplasmic reticulum, and cytoskeletal proteins using two-dimensional gel electrophoresis (2DE) method. The current study was designed to analyze changes in cellular and secreted proteins in rat BEC exposed to ischemic insult in vitro using two complementary quantitative proteomic approaches, 2DE and isotope-coded affinity tag (ICAT)-based proteomics. The study provides comprehensive qualitative and quantitative comparison between the two proteomic methods applied to the same experimental system with respect to their reproducibility and the type of proteins identified.'"

Definition
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