Semantic resources project/Antibodies/Revised Methods/Western Blot

Description
WB: Western Blot

Outline
Gel electrophoresis of proteins, followed by transfer to a membrane and probing with an antibody. The antibody is linked to an enzyme, which is used to provoke a visible colored reaction.

Ontology Terms
NCI Thesaurus: "Western Blotting"
 * http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#Blotting_Western
 * http://bioportal.bioontology.org/visualize/40377/Blotting_Western
 * "A laboratory procedure for the detection or identification of proteins or peptides that have been separated by gel electrophoresis and transferred onto nitrocellulose or other type of paper or nylon membrane. The proteins are then detected by reaction with enzymatically labeled or radiolabeled antibody probes."

MeSH: "Blotting, Western"
 * D015153
 * http://bioportal.bioontology.org/visualize/40401/D015153
 * "Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes."

Sample Processing and Separation Techniques: "western blot"
 * sep:00158
 * http://bioportal.bioontology.org/visualize/39509/sep%253A00158
 * Sees as a synonym for 'immunoblot'
 * "A western blot is a biological blot protocol which is used in molecular biology/biochemistry/immunogenetics to detect protein in a given sample of tissue homogenate or extract. It uses gel electrophoresis to separate denatured proteins by mass. The proteins are then transferred out of the gel and onto a membrane (typically nitrocellulose), where they are "probed" using antibodies specific to the protein."

Physico-chemical Methods and Properties: "Western blotting"
 * FIX:0000638
 * http://bioportal.bioontology.org/visualize/2991/FIX%253A0000638

HL7v3.0: "Western Blot"
 * 0138
 * http://bioportal.bioontology.org/visualize/40392/0138

Protein-protein interaction: "Western Blot"
 * MI:0113
 * http://bioportal.bioontology.org/visualize/39508/MI%253A0113
 * Sees Western and immunoblotting as identical (exact synonyms)
 * "Western blot is a procedure to identify and quantify proteins. A mixture of protein is first submitted to an electrophoresis in denaturing condition and then electro-transferred from the gel to a membrane. The membrane is then incubated with a primary antibody specific for a given protein or a specific residue modification in the sample under analysis. A secondary antibody, radiolabelled or fused to fluorophore or to a chromogenic enzyme, targets the first antibody and allows the visualisation of the protein band on the membrane."

Definition
None yet