Semantic resources project/Antibodies/Revised Methods/Complex Immunoprecipitation

Description
Complex Immunoprecipitation
 * aka. Co-IP
 * aka. Coimmunoprecipitation

Outline
Involves "pull downs," purifying out a complex of proteins using an antibody which is specific to just a single component of the complex.

Subtypes may include:
 * anti-bait co-ip
 * anti-tag co-ip
 * flag tag
 * ha tag
 * his tag
 * myc tag
 * tap tag
 * "immunodepleted" co-ip

Ontology Terms
Most of the ontology terms for CoIP are (as we might expect) in the "protein-protein interaction" ontology.

PPI: "coimmunoprecipitation"
 * MI:0019
 * http://bioportal.bioontology.org/visualize/39508/MI%253A0019
 * In this approach an antibody, specific for the protein of interest (bait) or any tag expressed within a fusion protein, is used to separate the bait from a protein mixture or a cell lysate and to capture its ligand simultaneously. The protein partners that bind to the bait protein retained by the resin can then be eluted and identified.

Usage
Koffa et al. "HURP Is Part of a Ran-Dependent Complex Involved in Spindle Formation" Current Biology 16:8,743-754 (April 2006) "'From different purification attempts, it became obvious that mild, native conditions were needed to maintain the activity, suggesting that some of the identified proteins might be in a complex. Immunoprecipitation experiments confirmed this conclusion. HURP, TPX2, XMAP215, Eg5, and Aurora A were coimmunoprecipitated by several antibodies, although use of the Aurora A antibody appeared to disrupt the complex (Figure 1D).'"

Definition
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