Semantic resources project/Antibodies/Revised Methods/In-situ Hybridization

Description
ISH: in situ hybridization

Ontology Terms
MeSH: "In Situ Hybridization"
 * D017403
 * http://bioportal.bioontology.org/visualize/40401/D017403
 * "A technique that localizes specific nucleic acid sequences within intact chromosomes, eukaryotic cells, or bacterial cells through the use of specific nucleic acid-labeled probes."

NCI Thesaurus: "in situ Hybridization"
 * http://ncicb.nci.nih.gov/xml/owl/EVS/Thesaurus.owl#in_situ_Hybridization
 * http://bioportal.bioontology.org/visualize/40644/in_situ_Hybridization
 * "Use of a DNA or RNA probe to detect the presence of complementary sequences in chromosomes, cells, or tissues"

HL7 v3.0: "In-situ hybridization"
 * 0123
 * http://bioportal.bioontology.org/visualize/40392/0123

NIFSTD: "In-situ hybridization"
 * http://ontology.neuinfo.org/NIF/DigitalEntities/NIF-Investigation.owl#nlx_inv_20090610
 * http://bioportal.bioontology.org/visualize/40510/p17%253Anlx_inv_20090610

Usage
Jelsing, Larsen, and Vrang. Identification of cannabinoid type 1 receptor expressing cocaine amphetamine-regulated transcript neurons in the rat hypothalamus and brainstem using in situ hybridization and immunohistochemistry. Neuroscience 154:2,641-652 (June 2008) "'Using double in situ hybridization, co-localization between CART and CB1R mRNA expressing neurons was observed to be most pronounced in the retrochiasmatic and lateral hypothalamic areas, as well as in all parts of the dorsal vagal complex. Further attempts to immunohistochemically characterize the distribution of CB1R were, however, deemed impossible as any of eight commercially available antibodies/antisera gave rise to non-specific staining patterns.'"

Definition
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